Due to the enhanced stability, they can be shipped and stored at room temperature, reducing cost and improving accessibility globally. Just as protein encapsulation of viruses offers more stable molecular storage, Twist’s Encapsulated Control products leverage a novel method to seal a metal capsule around a desiccated pellet to enhance stability of the RNA controls. Twist Bioscience Encapsulated Control products offer a unique storage and delivery method for Twist’s established Synthetic SARS-CoV-2 RNA controls. The Twist Assay Ready Synthetic SARS-CoV-2 RNA Control is now available for customers outside the CDC. The Twist Assay Ready Synthetic SARS-CoV-2 RNA Control was developed in partnership with the US CDC and integrated into the CDC Influenza SARS-CoV-2 (Flu SC2) Multiplex Assay, which tests for influenza A, B and SARS-CoV-2 simultaneously. The Encapsulated Controls are supplied at approximately 50,000 copies per tube. The Assay Ready Controls are supplied at approximately two million copies per tube. Twist also offers certain controls in Assay Ready and Encapsulated Formats that are supplied as a desiccated pellet. The majority of the controls are provided in the standard format which consist of 100 μL at a concentration of approximately one million copies per microliter. These controls provide coverage of greater than 99.9% of the bases of the SARS-CoV-2 viral genome. Each control consists of six non-overlapping 5 kb fragments generated from Twist Gene Fragments then transcribed into ssRNA. These variants were selected to cover a wide range of sequence diversity.
Twist has created synthetic RNA controls for several viral genomes. Ambient shipping reduces cost and increases accessibility for researchers across the globe. The enhanced stability of the Encapsulated controls enables extended shelf life and both ship and store at room temperature. The Assay Ready format allows for shipping at ambient temperatures and storage in standard freezers. Just as protein encapsulation of viruses offers more stable molecular storage, Twist’s The Encapsulated Control products leverage a metal capsule sealed around a desiccated pellet to enhance stability of the RNA controls.īoth the Assay Ready and the Encapsulated formulations are single use controls. The Assay Ready Controls are provided as a dried pellet that increases stability. The standard format provides the controls at high concentration in a frozen liquid formulation. Synthetic controls created through gene synthesis broaden access across diverse strains while mitigating risk.Ĭhoose from Standard, Assay Ready, or Encapsuled Control formulations.
#Gamma control v4 verification
This includes the verification and validation of diagnostic tests of both next-generation sequencing (NGS) and reverse transcription polymerase chain reaction (RT-PCR) assays.Ī Safe and Effective Alternative to “Live Virus”Ĭontrols based on viral nucleic acids extracted from either an infected patient or from live virus propagated in cell culture have safety and security concerns. Positive controls provide quality control measures for a wide range of applications from diagnostic assay development to day-to-day testing. To address this need, laboratories around the world need high quality tools to enable them in catering to this rapidly expanding requirement for testing. The recent Coronavirus pandemic has led to the unprecedented need for diagnostic tests for detecting the presence of SARS-CoV-2 and other Respiratory viruses in a variety of sample types.
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